Fig. 6
Porcine sperm bound to suLeX coverslips pre-fertilization successfully fertilize oocytes in vitro. IVF was performed under conventional conditions (Control; 5 h with 0.1 × 106 sperm/mL) and on suLeX coverslips with sperm bound pre-fertilization (glycan-IVF; 5 h with 0.25 × 106 sperm/mL). Pronuclear formation was evaluated 12 h after the beginning of IVF by Hoechst staining. (a) Frequency of fertilized oocytes from total cultured oocytes, (b) Frequency of polyspermic zygotes from total fertilized oocytes, and (c) IVF efficiency or frequency of monospermic zygotes from total cultured oocytes. The error bars indicate mean ± SEM.
