Fig. 2

Phylogenetic tree of NAC proteins from various plant species, including Arabidopsis thaliana (27), Oryza sativa (20), Hordeum vulgare (26), Triticum aestivum (19), Zea mays (23), and Brachypodium distachyon (14). The tree was constructed using the Neighbor-Joining method implemented in MEGA 11, based on protein sequences aligned with ClustalW. Bootstrap values (1000 replicates) are shown at the nodes to indicate the support for each branching point. Distinct clades are color-coded to highlight the evolutionary relationships and diversification among the NAC proteins from these species.