Fig. 3 | Scientific Reports

Fig. 3

From: Bafilomycin A1 induces colon cancer cell death through impairment of the endolysosome system dependent on iron

Fig. 3

BA1 increased size of perinucleic acidic vesicles independent of endolysosomal pH. Cells were treated with BA1 (LoVo: 2 nM, SW480: 1.5 nM) with or without iron (III)-citrate. (A) Representative confocal image of cells stained with acridine orange. Orange-red fluorescence indicates acidic compartments. Scale bar: 10 μm. (B) Cells were stained with LysoTracker Red DND-99, and pH-sensitive probe (BHEt-1). (C) Quantitative intracellular pH was measured using LysoSensor Yellow/Blue DND-160 by a microplate reader. Scale bar: 10 μm. #p > 0.05 (no significance), *p < 0.05, **p < 0.01, ***p < 0.001, compared with control.

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