Fig. 7
BA1 induced ER stress. Cells were treated with BA1 (1.5 or 2 nM) with or without iron (III)-citrate. (A,B) Expression of the ER stress markers detected by western blotting (A), and RT-qPCR (B). (C) Detection of XBP1 splicing by PCR analysis. Tunicamycin treatment was used as a positive control for inducing ER stress. (D) Representative images were captured by laser confocal microscope after staining with ER-Tracker Blue-White DPX. Scale bar: 10 μm. #p > 0.05 (no significance), *p < 0.05, **p < 0.01, ***p < 0.001, compared with control.
