Table 1 Characteristics of liver donors.

Sample #	Sex	Age	Known consumption^a	UGT2B17 genetic status (# gene copies)^b	UGT2B17 peptide quantification (pmol/mg protein)^c	Relative UGT2B17 quantification (× 10⁵ LFQ)^d
1	F	66	S	0	0.1	0
2	F	7		0	0.1	0.005
3	M	74	A	0	0.0	0
4	M	58	S	0	0	0
5	F	41		2	20.0	2.1
6	M	76	S, A	1	23.5	4.8
7	F	77		0	0	0
8	M	59	A	1	29.1	3.9
9	F	77		0	0.2	0.9
10	M	69	A	1	20.1	5.0
11	F	45		2	5.9	0.3
12	F	56	S, A	1	13.5	2.9
13	F	37		2	4.5	0.3
14	F	47		1	34.4	11.4
15	F	47		0	0	0

^aA Alcohol intake; S Smoking.
^bComplete germline UGT2B17 gene deletion: 0; One or two UGT2B17 gene copies: 1 or 2. Determined by qPCR and immunoblotting with a UGT2B17 specific monoclonal antibody as described.¹⁹ The gene-proficient group was composed of individuals with one or two gene copies given the documented minor gene-dose effect on UGT2B17 expression and activity.^8,19,24.
^cQuantification by mass spectrometry using a UGT2B17 signature peptide, in hepatic microsomal fractions from the same liver donors, as previously reported.²³.
^dThis study; relative label-free quantification (LFQ) based on unique UGT2B17 peptides (see Methods section and Supplementary Table 1). The Spearman correlation between quantification by the two MS approaches was elevated (r = 0.953, P = 0.0001).

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