Fig. 2 | Scientific Reports

Fig. 2

From: Retarding human adipose-derived MSCs senescence and promoting tendon repair using cell sheet engineering with a histone methyltransferase inhibitor

Fig. 2

A gradual increase of SETD7 was observed during successive passages of hAD-MSCsin vitro. (A) SA-β-gal staining in serial passage hAD-MSCs (red arrows: senescent cells). Scale bar = 50 μm. (B) The percentages of senescent cells were determined. (C) SETD7 and senescence-associated gene expression were assessed by qRT-PCR in hAD-MSCs at P4, P8, and P10, n = 3. (D, E) Western blot and quantitative analysis of SETD7, p21, and p16INK4A protein expression in hAD-MSCs at P4, P8, and P10, n = 3. Data shown from representative sample and represented as mean ± SD. One-way analysis of variance (ANOVA), *p < 0.05, **p < 0.01. Abbreviation: P, passage.

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