Fig. 3

SDS-PAGE analysis of the overexpressed and purified enzyme (12.5% polyacrylamide gel). Lane 1 shows molecular weight markers, and lanes 2 and 3 show purified UOX mutant using Ni–NTA affinity chromatography.
SDS-PAGE analysis of the overexpressed and purified enzyme (12.5% polyacrylamide gel). Lane 1 shows molecular weight markers, and lanes 2 and 3 show purified UOX mutant using Ni–NTA affinity chromatography.