Fig. 1 | Scientific Reports

Fig. 1

From: Comparative analysis and process optimization for manufacturing CAR-T using the PiggyBac system derived from cryopreserved versus fresh PBMCs

Fig. 1

Comparison of cell viability and phenotype of cryopreserved versus fresh PBMCs. (A) Experimental workflow. (B) Viability of PBMCs (n = 5–14). (C) Phenotypic analysis (n = 4). (D) The logic diagram for flow cytometry analysis. (E) Differentiation state of T cells (n = 4). (B) Median with 95% Confidence Interval (CI). (C), (E) Individual data and average. Statistical analysis was conducted using the Kruskal–Wallis test (B) and the Friedman test (C, E), with a p < 0.05 considered statistically significant. * p < 0.05, ** p < 0.01, “ns” indicates no significant difference.

Back to article page