Fig. 2

Comparison of quality attributes of mesoCAR-T derived from cryopreserved and fresh PBMCs. (A) Proportions of CD3⁺, CD3⁺CD4⁺, and CD3⁺CD8⁺ cells post magnetic bead enrichment (n = 4). (B) Structure of the mesoCAR. Viability (C) and cell expansion potential (D) during mesoCAR-T manufacturing (n = 4). The histogram in (C) and (D) represent the viability and amplification of CAR-T cells on the 11th day of culture, respectively. (E) Percentage of CD3⁺, CD4⁺, CD8⁺ and mesoCAR⁺ cells (n = 4). Comparative analysis of the differentiation states (F) and exhaustion (G) (n = 4). The histogram in E–G present the statistical analysis of the CAR-T product. (H) Toxicity capacity curves, tested at various E:T (4:1 or 2:1) against SKOV-3 cells within 24 h (n = 4), and the toxicity at 24 h was analyzed statistically. (I) Cytokine secretion (n = 4). The data presented in (A) includes both individual and average values, whereas (C-I) show median with 95% CI. Dots of the same shape represent data from one individual. The Kruskal–Wallis test (A) and the Friedman test (C-I) were used for statistical analysis, with a p < 0.05 was considered significant. *p < 0.05, “ns” indicates no significant difference.