Fig. 1

BRISC is upregulated in liver of ASH patient and mouse model. (A) ABRO1 and BRCC3 expression levels in the liver samples of patients with alcoholic hepatitis from published transcriptome dataset (GSE28619). (B-D) WT mice were subjected to chronic-plus-binge ethanol feeding for 2, 4, 6 weeks (N = 3–6). Representative liver H&E staining images were shown; Scale bar, 100 μm (B). Steatosis were quantified by measuring liver triglyceride content (C). Western blot analysis of ABRO1 and BRCC3 levels in total liver lysates (D). (E, F) WT mice were subjected to chronic-plus-binge ethanol feeding for 10 days (N = 3). Expression of BRISC components was detected by western blot (E) and real-time PCR (F). Data are presented as mean ± SEM; expression scores are shown as box plots, with horizontal lines representing the median; the bottoms and tops of the boxes represent the 25th and 75th percentiles, respectively, and the vertical bars represent the range of data; *P < 0.05, **P < 0.01, ***P < 0.001; two-tailed unpaired t-test.