Fig. 2

ABRO1 deficiency ameliorates chronic-plus-binge ethanol-induced mice liver injury. WT and Abro1^−/− mice were subjected to chronic-plus-binge ethanol feeding for 10 days (N = 4–9). (A) Liver samples were stained with H&E or ORO, and ORO-positive areas were quantified. Scale bar, 100 μm. (B, C) Liver injury and steatosis were quantified by measuring serum ALT and hepatic triglyceride content. (D) Relative mRNA levels of hepatic pro-inflammatory cytokines. Immunohistochemistry (IHC) staining analysis of F4/80⁺ cells (E), Ly6G⁺ cells (F), and4-HNE expression (G) in the liver. Representative IHC images and quantification of positive areas were shown. Scale bar, 50 μm. Data are presented as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001; two-tailed unpaired t-test.