Fig. 3
Protein analysis of cortical tissue: (A) Western Blotting protocol showing LRP5 staining in the cortical tissue of control and FCD. (B) Western blot gel showing corresponding bands for β-catenin and phosphorylated β-catenin. (C) Quantification of β-catenin and phosphorylated β-catenin in cortical tissues from control and FCD samples. (D) The percentage of phosphorylated β-catenin, determined by the difference between total and phosphorylated β-catenin, showed a lower amount of phosphorylated β-catenin in FCD samples (p = 0.008), indicating increased WNT pathway activity. The asterisks above the graphs indicate statistical significance as follows: *0.01 < p < 0.05, **0.001 < p < 0.01, ***p < 0.001. The t test was used to determine statistical significance. Error bars represent the standard error of the mean (SEM).
