Fig. 5
From: Perinatal obesity primes the hepatic metabolic stress response in the offspring across life span
Offspring exposed to perinatal obesity display an increased oxidative stress level in early life which normalizes in late adulthood. (a, b) Western blot analysis of 4-Hydroxynonenal (4-HNE) as a marker of lipid peroxidation shows an increased oxidative stress level in early life that diminishes until late adulthood. Cropped images are shown with their respective loading control. (c, d) Malondialdehyde (MDA) as another marker of lipid peroxidation shows no alterations in early life or late adulthood. (e–g) Western blot analysis of complex I-V of oxidative phosphorylation. Analysis of complex II-V confirms a decreased oxidative phosphorylation in early life that normalizes in late adulthood. Complex I did not show up in all western blots. Representative blots are displayed with their respective loading control. The cropped images in (f) are shown at different exposure times for demonstration purposes. Uncropped images of original blots at the same exposure time are provided in Supplementary Fig. S5 (n = 5 for all groups). Data are presented as mean ± SEM. T-Test and Mann–Whitney-U-test were used and statistical significance was considered as p < 0.05 and marked as *.
