Fig. 5

Cell viability and migration assays studies on MDA-MB-231 cells. (A) Viability of MDA-MB-231 cells treated with SLN, C-peptide, PTX, SLN-PTX and C-peptide-SLN-PTX at concentrations equivalent to PTX (0.5, 1, 2, 4, 6, 8, 10, 12, 14, 16 µg/ml) after 24 h. The MTT assay was utilized to assess cell viability. The data were analyzed using Prism 8 software, applying a two-way ANOVA. The results are presented as mean ± SEM, with significance levels indicated as follows: ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, and ns for not significant (n = 3). (B) The scratch migration assay was conducted on MDA-MB-231 cells treated with various conditions: PBS (control), SLN, PTX, C-peptide, SLN-PTX, and C-peptide-SLN-PTX. Cells were incubated for durations of 0, 24, and 48 h. C: Wound areas were quantified using ImageJ and Prism 8 software. The analysis employed a two-way ANOVA, with results expressed as mean ± SEM. Significance levels were determined as follows: ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, and ns for not significant (n = 3).