Fig. 10
ADAM12 promotes the malignant progression of glioma cells. (A) Protein levels of ADAM12 in six pairs of glioma tissues (T) and adjacent non-tumor tissues (N).Full-length original Western blots are presented in Supplementary Fig S5A. (B, C) Validation of siRNA interference efficiency in knockdown of ADAM12 expression in U87 and U251 cells by RT-qPCR and Weston blotting.Full-length original Western blots are presented in Supplementary Fig S5B. (D) CCK8 assay to detect the proliferative capacity of transfected U87 and U251. (E, F) Wound healing assay for negative control and ADAM12 knockout groups. (G) Colony formation assay for negative control and ADAM12 knockout groups. (H, I) Transwell assay images of migration and invasion in the negative control and ADAM12 knockout groups. *p < 0.05; **p < 0.01; ***p < 0.001.
