Fig. 4

Alterations in brain transcriptome and serum metabolome of mice subjected to 3D clinostat. (a) Venn diagram illustrating differentially expressed genes between pairwise comparisons; (b) Heatmap of differentially expressed genes exhibiting significant differences between the CS and SB groups; (c) Main KEGG pathways affected between the CS and SB groups; (d) OPLS-DA analysis of serum metabolites between groups; (e) Upregulated and downregulated differential serum metabolites between the CS and SB groups; (f) KEGG analysis of serum metabolites revealing the main pathways affected between the CS and SB groups. MC: ordinary cage control group; SB: survival box control group; CS: 3D clinostat model group.