Fig. 6

Comparison of the 3D clinostat simulated microgravity effect model with the classical hindlimb unloading model and spaceflight mice. (a) Venn diagram of differentially expressed genes in brain transcriptomes among the three models; (b) Main KEGG pathways affected in the hindlimb unloading model; (c) Main KEGG pathways affected in the spaceflight model; (d) Heatmap visualizing the expression levels of 131 genes commonly detected in all three models and fulfilling the criteria of differential expression (P value < 0.05 and fold change > 2 or fold change < 0.5) in at least one model. Red markers: KEGG pathways commonly affected by all three models; Green markers: KEGG pathways commonly affected by the HU and FL models; Blue markers: KEGG pathways commonly affected by the CS and FL models. CS: 3D clinostat model; HU: hindlimb unloading model; FL: spaceflight model.