Fig. 4
Analysis of lung fibrosis inTerc mutant mice. PanelA. Expression of αSMA in lungs of CT-Terc (upper panel) and C57-Terc (lower panel) mutant mice analyzed by immunofluorescence and quantified by image analysis (Left panels). Representative images of αSMA expression are shown at the right panels. The green signal showed the αSMA protein expression and the pink signals showed cell nuclei stained by TO-PRO-3. Data are presented as mean ± SD. **p < 0.01 vs. CT-Terc+/+ −2 m (one-way ANOVA and Dunnett´s comparison test). Magnification 40x. Panel B. Determination of lung fibrosis by Masson’s trichrome staining. Upper panel, representative photographs of lung sections stained with Masson trichrome, collagen type I fibers appear blue, muscle fibers and cytoplasm appear red and the nuclei appear blue-black. Magnification 40x. Lower panels, quantification of lung collagen deposition in CT-Terc (left panel) and C57-Terc (right panel) mice. Data are presented as mean ± SD and analyzed by ANOVA and Dunnett´s comparison test. *p < 0.05 vs. CT-Terc+/+ 2 m animals.
