Fig. 5
Analysis of alveolar type 2 cells in the lung ofTerc mutant mice at different ages. Panel A. Quantification of alveolar type 2 cells in lungs from the different mice strains and genotypes. The number of Pro-SPC cells was normalized to the number of nuclei in each field. Right panels, representative photographs of lung sections. The green signal showed the pro-SPC protein expression and the pink signals showed cell nuclei stained by TO-PRO-3. Magnification 40x. Quantification of the presence of alveolar type 2 cells in CT-Terc (upper panel) and C57-Terc (lower panel) mice. Panel B: Immunohistochemistry analysis for p21 expression in lung sections from CT-Terc and C57-Terc mice at 2 and 10 months of age. Representative images (left panels) and quantification (right panels) of p21 positive cells. Magnification 40x. Arrowhead point to p21-positive cells (brown color). Quantifications were performed on ten random, different areas of each preparation. Data are presented as mean ± SD and analyzed by ANOVA and Dunnett´s comparison test. *p < 0.05 and **p < 0.01 (vs. wild type mice at 2 months of age).
