Fig. 2 | Scientific Reports

Fig. 2

From: Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages

Fig. 2

Fluorescence microscopy images showing the different morphologies of boMdM 24 h after stimulation with (a) PBS (Control), (b) non-EV secretions, (c) SaEVs, and (d) LTA. Different magnifications illustrate the typical morphology of non-stimulated cells, the abundance of morphological changes, and specific cytoplasmic changes in response to stimuli. Actin cytoskeleton was stained with phalloidin (blue), and DNA was stained in DAPI (purple). White arrows point to those boMdM with morphological changes. (e-g) Additional fluorescence microscopy images were acquired at a higher magnitude to appreciate the cytoplasmatic changes in boMdM.

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