Fig. 3

Effect of M5512B SaEVs on bovine Monocytes derived Macrophages (boMdM). Relative gene expression of pro-inflammatory genes (a), anti-inflammatory genes (b), and apoptosis-related genes (c) in boMdM 24 h after stimulation with PBS (control), non-EV secretion, SaEV, or LTA. Bar charts show the mean and standard deviation. Groups with different letters are statistically different from each other at a significance level of P < 0.05. “ns” denotes no significant differences. (d) Interleukin 1B protein release in boMdM 24 h after stimulation with PBS (control), non-EV secretion, SaEVs, or LTA.