Fig. 6

IPA alleviated the apoptosis of HCs and SGNs induced by TCP. (A) TUNEL staining results of HCs in the basal turn of cochlear basilar membrane in the group of NC, 40 mg/kg IPA, 50 mg/kg TCP, 20 mg/kg IPA + 50 mg/kg TCP, and 40 mg/kg IPA + 50 mg/kg TCP after 21 days of gavage (corn oil was used as a solvent, n = 3), (B) TUNEL staining results of SGNs in the basal turn of the cochlear basilar membrane in the group of NC, 40 mg/kg IPA, 50 mg/kg TCP, 20 mg/kg IPA + 50 mg/kg TCP, and 40 mg/kg IPA + 50 mg/kg TCP after 21 days of gavage (corn oil was used as a solvent, n = 3), (C,D) Results of TUNEL-positive cell counts after 21 days of gavage in NC, 40 mg/kg IPA, 50 mg/kg TCP, 20 mg/kg IPA + 50 mg/kg TCP, and 40 mg/kg IPA + 50 mg/kg TCP groups (Corn oil as solvent, n = 3); Bar = 50 μm; Data expressed as a percentage; ***: p < 0.001 compared with control; ****: p < 0.0001 compared with control; ##: p < 0.01 compared with 50 mg/kg TCP group; ###: p < 0.001 compared with 50 mg/kg TCP group; ####: p < 0.0001 compared with 50 mg/kg TCP group by one-way ANOVA with Bonferroni post hoc test.