Table 3 Phytochemical screening of seed and oil extracts in fenugreek.

From: Fenugreek seeds as a natural source of L-arginine-encapsulated lipid nanoparticles against diabetes

Sr #.

Screening

test

Screened

phytochemical

Inference

Result

Extract

Oil

1.

Wagner’s Test

Alkaloids

Appearance of reddish brown color with Wagner’s reagent

+++

+++

2.

Foam Test

Saponins

Formation of stable foam

+++

+++

3.

Ferric Chloride Test

Phenols

Indication of blue green color with ferric chloride

+++

+++

4.

Braymer’s Test

Tannins

Formation of green precipitates

---

---

5.

Salkowski’s Test

Terpenoids

Appearance of yellow color

---

---

6.

Bontrager’s Test

Quinones

Occurrence of red color in alkaline phase

+++

+++

7.

Keller Killani’s Test

Cardiac Glycosides

Formation of pink to blood red coloration

---

---

8.

Glycosides Test

Glycosides

Indication of pink color

+++

+++

9.

Alkaline Reagent Test

Flavonoids

Formation of yellow color which becomes colorless on addition of acid

+++

+++

10.

Precipitate Test

Phlobatannins

Appearance of red precipitates

---

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