Fig. 2

scRNA-seq reveals 11 clusters of hNTSCs. (A) Overview of the experimental procedure. Human NTSCs were subjected to single-cell suspension, library construction, and sequencing. (B) Workflow for data analysis using CellRanger pipeline and Seurat analysis. (C) Raw data (n = 23,456 cells) were clustered and UMAP projection of 11 clusters of 4 donor-derived hNTSCs was performed. (D) Representative mesenchymal stem cell marker expression of 4 donor-derived hNTSC aggregates. x axis: cell clusters C0 to 10, y axis: surface markers (E) Functional annotation analysis for significant probes was performed using g: Profiler.