Table 2 Developed SSRs loci, repeat motifs, primer sequences, size range, annealing temperatures and heterozygous individuals numbers.
Loci | Sequence (FP/RP) | Repeat motif | Size range | Annealing temperature (°C) | No. of samples amplified | Heterozygous individuals |
|---|---|---|---|---|---|---|
CsM43755 | GCAGAGCCAGGCTTACAAA | (TTA)10 | 227 | 54 | 96 | 46 |
GTCGTTGCCTGTACATTTG | ||||||
CsM29435 | ATGCAGCCTTGCTCGATAGT | (ATT)10 | 212 | 55 | 88 | 0 |
TTTTTGTACTGGGTCAAGTGG | ||||||
CsM45955 | TGCCTCAACACAATATCCTTCA | (AATA)9 | 196 | 54 | 97 | 13 |
TGGACCAAGTTCTTAGTCTGACA | ||||||
CsM81952 | TTCCCTACACGACGCTCTTC | (CAAG)9 | 198 | 53 | 60 | 12 |
AGCCATTCATCCAAAAATGC | ||||||
CsM48497 | TACACCGCGGCACTAATGTA | (ATTT)8 | 192 | 53 | 60 | 40 |
CAAACTGTGAATGCTGATTTGG | ||||||
CsM30177 | GTTAAGCCGGAACATGGTGA | (CACGT)7 | 257 | 52 | 96 | 44 |
TCACGTGGAGTAACGTGACATA | ||||||
CsM37073 | TGCCATGCTCTTGCAAAATA | (AAT)6aac(AAT)5 | 228 | 54 | 97 | 50 |
CGATAGTGACTTTGTTGAGACTCC | ||||||
CsM38056 | TATGCCATGCTCCAGCTTC | (GCTTCG)5 | 174 | 52 | 92 | 12 |
TCATTTTCAAGGACGAACTC | ||||||
CsM12479 | GGACCAGATGCGTGTGTTAG | (TATT)9 | 269 | 55 | 96 | 44 |
CCTCTGTTATTGGGTGTCCTG | ||||||
CsM92789 | GCGCATAGAAGCACTACTTTCA | (AC)11aag (AG)11 | 160 | 55 | 96 | 36 |
TCATTCTCCTCCACTTCACAGA |
