Fig. 3

Nanozein reduces intracellular ROS levels without affecting cell viability. HEY and COV362 cell viability was assessed through propidium iodide (PI) staining following treatments with increasing amounts (5, 10, 20 and 40 µg/ml) of Nanozein for 24 h. The percentage of dead cells (PI positive) are reported in each plot (plots are representative of single experiments) (a). CMH₂DCFDA cytofluorimetric assay was carried out. The intracellular ROS levels decreased after incubation with Nanozein for 24 h (HEY and COV362 cells were treated with 5 µg/ml and 10 µg/ml of Nanozein, respectively) (b). Histogram values are expressed as mean ± standard deviation of three biological replicates (c): *p < 0.05, **p < 0.01 as compared to non-treated cells. All the experiments were performed in triplicate.