Fig. 10

Effects of SZDTD on AMPK and PI3K signaling pathways in in vitro assays. (A) The result of MTT assay to detect the cellular value-added situation. (B) The production of AMPK, P- AMPK, SIRT1 and PGC-1α in the BEAS-2B human bronchial epithelial cell line was estimated by western blotting, and the relative protein expression (C) was measured. β-Actin and AMPK are on the same gel, P-AMPK and PGC-1α are on the same gel, SIRT1 is on a separate gel. *P < 0.05 vs CON group. #P < 0.05vs LPS group. (D) The production of PI3K, P-PI3K, AKT, P-AKT in the lung homogenates was estimated by western blotting, and the relative protein expression (E) was measured. All lanes are separate gels. *P < 0.05 vs CON group. #P < 0.05vs LPS group.