Fig. 5 | Scientific Reports

Fig. 5

From: Exosomes derived from hypoxic mesenchymal stem cell ameliorate premature ovarian insufficiency by reducing mitochondrial oxidative stress

Fig. 5

NExos and HExos accelerated KGN cell propagation and attenuated CTX-induced KGN cell apoptosis. (A) Uptake of the green fluorescence dye Dio labeled NExso and HExos into KGN cells (Scale bar = 50 μm). (B) Detection of cell apoptosis of different groups by flow cytometry. (C) Detection of cell proliferation curves by CCK-8 assay. (D) Detection of mRNA levels of caspase 3 in the ovary of different rat groups. (E) Detection of mRNA levels of caspase 9 in the ovary of different rat groups. (F) Apoptosis protein expression analysis in the ovary of different rat groups. (G) The histogram summarizes the protein expression levels. Three distinct experiments were evaluated via Student’s t-test, with error bars illustrated as means ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001).

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