Fig. 5

Interaction between normally raised KM mice and mice from the same or other strains reveals discrepancy only in PAG neuronal activity. (A) c-Fos neuronal fluorescence staining images with a scale of 1:100 μm. Ctl represents the control group, and the treatment groups include the same-strain (SS) and other-strain (OS) social interaction groups. Brain region localization: PFC (Bregma: 1.78 mm to -1.34 mm), BLA (Bregma: -1.34 mm to -1.58 mm), PIL (Bregma: -3.16 mm to -3.40 mm), SUM (Bregma: -2.80 mm to -3.08 mm), CA2 (Bregma: -2.92 mm to -3.08 mm), PAG (Bregma: -3.08 mm to -3.28 mm). (B) Statistical analysis of the number of c-Fos⁺ cells. The x-axis represents the control group and treatment groups, and the y-axis represents the count of c-Fos expressing neurons (ANOVA, PFC: F _{(2, 22)} = 3.778, p = 0.0389, n: Ctl = 8,SS = 8, OS = 9; BLA: F _{(2, 18)} = 4.302, p = 0.0297, n: Ctl = 7, SS = 7, OS = 7; PIL: F _{(2, 20)} = 2.830, p = 0.0828, n: Ctl = 7,SS = 8, OS = 8; SUM: F _{(2, 20)} = 4.287, p = 0.0282, n: Ctl = 7, SS = 8, OS = 8; CA2: F _{(2, 21)} = 6.170, p = 0.0078, n: Ctl = 8,SS = 8, OS = 8; PAG: F _{(2, 18)} = 8.828, p = 0.0021, n: Ctl = 7,SS = 7, OS = 7). Data are presented as means ± s.e.m. Statistical significance was determined using One-way ANOVA, Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01.