Fig. 8
From: Neural mechanisms underlying strain preference behaviour and plasticity in mice
Effect of muscimol infusion into the PAG on social preference in C57 mice and assessment of anxiety- and depression-like behaviors. (A) Experimental timeline for C57 mice, including cannula implantation at 6 weeks of age, a two-week recovery period, and behavioural testing conducted 40 min after drug administration. (B) Strain social interaction test comparing social preferences between the saline control group and the muscimol-treated group (Paired t test, saline SS vs. saline OS: t5 = 3.468, p = 0.0179; muscimol SS vs. muscimol OS: t5 = 1.421, p = 0.2144. Unpaired t test, saline SS vs. muscimol SS: t10 = 0.008162, p = 0.9936; saline OS vs. muscimol SS: t10 = 2.236, p = 0.0493. n = 6). (C) Elevated plus-maze test assessing anxiety-like behaviour in C57 mice following muscimol administration (Unpaired t test, close arm: t10 = 1.276, p = 0.2310; open arm: t10 = 0.9844, p = 0.2310; center area: t10 = 0.4542, p = 0.6594; n = 6). (D) Forced swim test evaluating depression-like behavior in C57 mice post-muscimol treatment(Unpaired t test, Latency to immobile time (s), t10 = 0.1979, p = 0.8471; Immobile time(s), t10 = 0.8698, p = 0.4048; n = 6). (E) Verification of cannula placement and example of drug infusion state (scale bar: 1:200 μm). Data are presented as means ± s.e.m, *p < 0.05.
