Fig. 11

Igfbp3 protein weakened NAR anti-aging and anti-degeneration capacity. (A) WB assay of recombinant IGFBP3 protein on Col2a1, Mmp13, P21 and P16 proteins. (B) Immunofluorescence detection of the effect of recombinant IGFBP3 protein on Aggrecan. Scare bar = 50 μm. (C) Effect of recombinant IGFBP3 protein on SA-β-Gal activity. Scare bar = 100 μm.