Fig. 6 | Scientific Reports

Fig. 6

From: Efficacy of Naringenin against aging and degeneration of nucleus pulposus cells through IGFBP3 inhibition

Fig. 6

Knockdown of Igfbp3 alleviates IL-1β-induced senescence and degeneration of NPCs. (A) qRT-PCR analysis of Igfbp3 expression levels after transfection of NPCs with siRNA. (B) WB analysis of Igfbp3 expression levels after transfection of NPCs with siRNA. (C) Changes in the ECM markers Col2a1 or Mmp13 and the senescence markers P21 or P16 after transfection with siRNA were confirmed by WB. (D) Effect of transfection with siRNA on the proliferation of NPCs. Scare bar = 200 μm. (E) Effect of transfection with siRNA on the SA-β-Gal activity of NPCs. Scare bar = 100 μm.

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