Table 1 Oligonucleotide primer sequences used for the detection of various blood borne pathogens in the blood samples of wild lizards collected during present study.

Haemogregarines

18 S rDNA gene

5′GTT TCT GAC CTA TCA GCT TTC GAC G3′

5′CAAA TCT AAG AAT TTC ACC TCT GAC3′

600 bp

¹⁶

Toxoplasma gondii

First internal transcribed spacer (ITS-1) of ribosomal DNA

5′AGTTTAGGAAGCAATCTGAAAGCACATC3

5′GATTTGCATTCAAGAAGCGTGATAGTAT3′

300 bp

¹⁷

Plasmodium spp.

Cytochrome b gene

PMF 5′-CCTCACGAGTCGATCAGG-3′

PMR 5′-GGAAACCGGCGCTAC-3′

377–379 bp

¹⁸

Haemoproteus spp.

Cytochrome b

5’ ATT GGA TGT CAA TTA CCA CAA TC 3’

5’ GGG AAG TTT ATC CAG GAA GTT 3’

525–533 bp

¹⁸

Leucocytozoon spp.

COX1 gene

5′TGGAACAATAATTGSATTATTTACAYT − 3′

5′AACATATCATATTCCATCCATTTAGATTA − 3′

218 bp

¹⁸

Schellackia spp.

ssr RNA gene

5’ GGTTGATCCTGCCAGTAGT 3’

5’ GGAAGGAACCGGAAGAATGC 3’

706 bp

¹⁹