Fig. 5 | Scientific Reports

Fig. 5

From: VHL ameliorates arecoline-induced oral submucosal fibrosis by promoting HDAC6 ubiquitination and blocking NF-κB pathway

Fig. 5

VHL regulated oral submucosal fibrosis by modulating HDAC6. (A) The mRNA expression of VHL and HDAC6 was measured by qPCR. (B) The protein expression of VHL, HDAC6, p-IκBα, IκBα, p-p65, p65 was detected by WB. (C) The levels of IL-6 and TNF-α were measured by ELISA. (D) Effects of VHL and HDAC6 on arecoline -induced collagen gel contraction in BMFs expressing NC, oe-VHL, oe-VHL + HDAC6 and oe-VHL + BAY 11-7082. (E) Representative images of the wound area in BMFs expressing NC, oe-VHL, oe-VHL + HDAC6 and oe-VHL + BAY 11-7082. (F) The expression levels of fibrotic-associated proteins (α-SMA, COL1A1 and FN1) were analyzed by WB. *P<0.5, **P<0.01, ***P<0.001.

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