Table 2 The detailed information of antibodies used in this study.
From: Impact of LIN7A silencing on U87 cell invasion and its clinical significance in glioblastoma
Antibody type | Specificity | Dilution | Catalog number | Supplier |
|---|---|---|---|---|
Primary antibody | Anti-LIN7A (rabbit polyclonal) | WB 1:1000 IF 1:500 | PA5-30871 | Invitrogen |
Primary antibody | Anti-beta-catenin (rabbit polyclonal) | WB 1:4000 IF 1:2000 | ab6302 | Abcam |
Primary antibody | Anti-lamin B1 (rabbit polyclonal) | WB 1:1000 | ab16048 | Abcam |
Primary antibody | Anti-alpha tubulin (rabbit monoclonal) | WB 1:1000 | ab52866 | Abcam |
Primary antibody | Anti-human nestin (rabbit polyclonal) | IF 1:200 | ZRB69 | MilliporeSigma |
Primary antibody | Anti-isocitrate dehydrogenase 1 (IDH1)-R132H (mouse monoclonal) | IF 1:500 | SAB4200548 | Merck |
Secondary antibody | Alexa fluor 488 goat anti-rabbit IgG (H + L) | IF 1:500 | A-11008 | Invitrogen |
Secondary antibody | Alexa fluor 594 goat anti-mouse IgG (H + L) | IF 1:500 | A-11005 | Invitrogen |
