Fig. 1 | Scientific Reports

Fig. 1

From: Enhancing radiation-induced reactive oxygen species generation through mitochondrial transplantation in human glioblastoma

Fig. 1

Use of cell-penetrating peptides (CPPs) to increase mitochondria fraction in human U3035 GBM cells. Representative images of native U3035 cells (B,C) were compared to U3035 cells receiving 100 µg of isolated and MITO Green labelled mitochondria (1X; D,E) and 300 µg of isolated and MITO Green labelled mitochondria (3X; F,G). 10X magnification with 200 µm scale bar shown. ANOVA of mean intensities per cell analyzed (native n = 67; 1X n = 79; 3X n = 57) demonstrated statistical significance in differences across all groups (p < 0.001). Western blotting for cytochrome C in native U3035 cells and 1X and 3X U3035 mitochondrial transplants confirmed the increase in the mitochondrial protein in the transplants relative to the native cells (H). Original blot is presented in Supplementary Fig. S1.

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