Fig. 3
Quantification of mitochondrial density. Mitochondrial densities from each cell line were obtained by dividing the total mass of mitochondrial protein (ug) that were extracted from individual lines by the number of live cells following dissociation and mitochondrial isolation. To demonstrate the predictable dynamic nature of mitochondrial density, and intact mitochondrial signaling response in the U3020 and U3035 lines, mitochondrial densities were also included from cells cultured under hypoxic conditions. One-way ANOVA was conducted using a Tukey’s multiple comparisons pos-hoc test to determine significance in the difference of means between groups. All data were analyzed using * p < 0.05 and ** p < 0.01.
