Fig. 5

Fluorescent probe of ROS generation. Representative Images of: U3020, U3035, and U3035Mt20-3 × cells stained with CellROX™ Deep Red fluorescent ROS probe following 0 Gy X-ray irradiation (A–C). U3020, U3035, and U3035Mt20-3X cells stained with CellROX™ Deep Red fluorescent ROS probe following 8 Gy X-ray irradiation and assayed 1 h following treatment (D–F). U3020, U3035, and U3035Mt20-3X cells stained with CellROX™ Deep Red fluorescent ROS probe following 8 Gy X-ray irradiation and assayed 24 h following treatment (D–F). (G) Quantification of these data represented by mean fold-change of relative fluorescent units vs 0 Gy as a control.