Fig. 2
From: PTPRO represses breast cancer lung metastasis by inhibiting the JAK2-YAP axis
PTPRO suppresses EMT in breast cancer. (A) PTPRO expression inversely correlates with the EMT-related gene signature according to a gene set enrichment analysis plot (GSE19615; P = 0.036; NES, normalized enrichment score). (B) Representative images of immunohistochemistry detection of Ptpro, E-cad and N-cad in the tumours of Ptpro−/− PyMT mice (left). The bar charts show the quantification of the staining (right). (C) Representative immunohistochemistry images of PTPRO, E-cad and N-cad in the same tumours from two primary human breast cancer samples (left). Percentages of samples showing E-cad and N-cad expression relative to the level of PTPRO in 15 human breast cancer samples (right). (D) Western blotting was used to detect the expression levels of EMT-related markers in BT474 cells overexpressing PTPRO and ZR75-1-knockdown PTPRO cells, with β-actin as the internal reference. The error bars indicate the SEM. **P < 0.01, ***P < 0.001 by Student’s t test (B) or Spearman’s rank test (C).
