Fig. 4
From: PTPRO represses breast cancer lung metastasis by inhibiting the JAK2-YAP axis
PTPRO phosphatase activity is a determinant of JAK2–YAP pathway activation and the suppression of breast cancer metastasis. (A) Proteins extracted from BT474 cells transfected with vector, PTPRO, or PTPRO CS were analysed via Western blotting with antibodies against PTPRO, p-JAK2, p-YAP, E-cad, N-cad, and Vimentin; β-actin was used as an internal reference. (B) Representative images of BT474 cell migration and invasion (vector vs. PTPRO vs. PTPRO CS) (left). Scale bars: 100 μm. Quantitative analyses of cell migration and invasion (right). (C) Representative image of BT474 cell scratch healing (vector vs. PTPRO vs. PTPRO CS). The error bars indicate the SEM. ***P < 0.001, ****P < 0.0001 by one-way ANOVA with post hoc intergroup comparisons.
