Fig. 3 | Scientific Reports

Fig. 3

From: Redox profiling of preovulatory follicular fluid in the donkey is species-specific, and contributes to modulate sperm function

Fig. 3

Intracellular levels of ROS. (a) Total ROS, DCF+ intensity in viable sperm (PI−) and (b) superoxides (O2−), E + intensity in viable sperm (Yo-Pro-1−) in samples resuspended in TBM (TBM; blue line) and TBM supplemented with 20% preovulatory follicular fluid (TBM + FF; red line) at 0 min, and after 60 min and 120 min of incubation at 38 °C. Different superscript letters (a-b) indicate significant differences (P ≤ 0.05) between treatments at the same time point. Data are shown as mean ± SEM of nine independent replicates.

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