Table 4 Kinetic parameters assessed in sperm resuspended in TBM, and sperm resuspended in TBM supplemented with 20% PFF (TBM + FF) at 0 min, after 60 min and 120 min of incubation at 38 °C.

From: Redox profiling of preovulatory follicular fluid in the donkey is species-specific, and contributes to modulate sperm function

Time (min)

Treatment

Kinetic parameters (mean ± SEM)

VCL (µm/s)

VSL (µm/s)

VAP (µm/s)

LIN (%)

STR (%)

WOB (%)

ALH (µm)

BCF (Hz)

0

TBM

102.43 ± 4.60a

59.73 ± 4.57a

76.06 ± 4.67a

57.25 ± 3.39a

76.43 ± 2.33a

73.17 ± 2.47a

3.27 ± 0.12a

7.41 ± 0.20a

TBM + FF

89.22 ± 4.05a

54.01 ± 4.13a

69.11 ± 4.27a

59.80 ± 3.52a

76.93 ± 2.33a

76.41 ± 2.57a

2.85 ± 0.15a

7.01 ± 0.22a

60

TBM

70.93 ± 6.13a

43.07 ± 4.43a

53.31 ± 5.23a

58.09 ± 2.42a

78.79 ± 2.35a

72.80 ± 1.78a

2.92 ± 0.16a

6.77 ± 0.31a

TBM + FF

72.71 ± 3.95a

42.00 ± 3.74a

53.83 ± 3.86a

56.76 ± 2.96a

77.04 ± 2.83a

72.99 ± 1.93a

3.04 ± 0.12a

6.78 ± 0.29a

120

TBM

44.87 ± 4.05a

22.76 ± 3.24a

28.08 ± 3.03a

49.22 ± 3.49a

78.71 ± 3.52a

62,30 ± 2.50a

2.85 ± 0.19a

6.93 ± 0.85a

TBM + FF

64.93 ± 3.62b

36.80 ± 3.17b

46.55 ± 3.84b

54.79 ± 2.52a

77.29 ± 2.04a

70.13 ± 2.76b

2.86 ± 0.24a

6.84 ± 1.50a

  1. Curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), linearity coefficient (LIN), straightness coefficient (STR), wobble coefficient (WOB), amplitude of lateral head displacement (ALH), and beat-cross frequency (BCF). The different superscript letters (a-b) indicate significant differences (P ≤ 0.05) between TBM and TBM + FF at a given time point. TBM: sperm resuspended in TBM; TBM + FF: sperm resuspended in TBM supplemented with 20% of preovulatory follicular fluid. Data are shown as mean ± standard error of the mean (SEM) of nine independent replicates.