Table 2 Comparison of DNA sequencing and PCR–RFLP assay for identifying SNPs and mutations in Domains II and III of the voltage-gated sodium channel (vgsc) gene in Rhipicephalus linnaei from Thailand.

From: Mutations in the voltage-gated sodium channel associated with permethrin resistance in Rhipicephalus linnaei populations in Thailand

Domain of vgsc gene

Genotypes

Genotyping methods performed on individual tick

DNA sequencing

PCR–RFLP

II (c.190C > A/c.215G > T) (N = 48)

SS*/SS

14*

13

RS*/SS

6

7*

SS/RS

5

5

SS/RR

3

3

RR/SS#

3#

2

RR/RS#

3

4#

RR/RR

2

2

RS/RR

2

2

RS/RS

10

10

III (c.2134 T > C) (N = 28)

SS

14

14

RS

11

11

RR

3

3

  1. *, unmatched results of the individual sample at position 190 SNP, #, unmatched results of the individual sample at position 215 SNP. For the following SNPs/mutations: position 190: SS (c.190CC), RR (c.190AA or c.190AG), RS (c.190CA or c.190CG), position 215: SS (c.215GG), RR (c.215TT), RS (c.215GT), and position 2134: SS (c.2134TT), RR (c.2134CC), RS (c.2134TC).
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