Fig. 4 | Scientific Reports

Fig. 4

From: GOLPH3 promotes calcium oxalate-induced renal injury and fibrosis through Golgi stress-mediated apoptosis and inflammatory responses

Fig. 4

Deposited calcium oxalate stones regulates the PI3K/AKT/mTOR signaling in HK-2 cells (A) Western blots assay was used to detect the expressions of GOLPH3, Golgi structure and functional proteins (GRASP65, ARF4, PI4KIIIβ and MYO18A) and proteins related to renal fibrosis (Collagen I, Vimentin, α-SMA) in HK-2 cells. (B) Representative images of immunofluorescence staining of HK-2 cells GOLPH3 (red) and GM130 (green) (C) Expressions of PI3K/AKT/mTOR pathway-related proteins in HK-2 cells quantified by Western blotting. Data are the mean ± SEM of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001 versus NC group.

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