Fig. 5 | Scientific Reports

Fig. 5

From: GOLPH3 promotes calcium oxalate-induced renal injury and fibrosis through Golgi stress-mediated apoptosis and inflammatory responses

Fig. 5

Inhibition of GOLPH3 reduces apoptosis and inflammatory responses in Golgi stress-mediated HK-2 cells (A) Confirmation of siRNA-mediated GOLPH3 knockdown efficiency using immunoblotting in HK-2 cells. (B) The levels of TNF-α, IL-6, and IL-1β were determined by qPCR. (C, D) The expression of GOLPH3, Golgi structural and functional proteins (GRASP65, ARF4, PI4KIIIβ, and MYO18A) and renal fibrosis-related proteins (collagen I, waveform protein, and α-SMA) were detected by Western blotting and qRT-PCR in HK-2 cells in each group. (E) After treating HK-2 cells with 200 μg/mL COM for 24 h, the proportion of apoptotic HK-2 cells in each group was assessed by flow cytometry. (F) Expression of GOLPH3 in HK-2 cell was detected by immunofluorescence staining, and the percent of positive cells was quantified. (G) Western blotting was used to quantify the expression of PI3K/AKT/mTOR pathway-related proteins in HK-2 cells of each group. Data are the mean ± SEM of at least three independent experiments. **P < 0.01, ***P < 0.001,****P < 0.0001 versus Ctrl-siRNA group. #P < 0.01, ##P < 0.01, ###P < 0.001, ####P < 0.0001 versus Ctrl-siRNA + COM group.

Back to article page