Fig. 2
(a) Histograms obtained from flow cytometric analysis showing WI-38 cell cycle at 24 h after challenge with a dose of 2 Gy. (b) Reverse transcription-quantitative PCR results for the detection of CDK6 and Wee1 mRNA expression. (c) Representative flow cytometry dot plots of WI-38 cells after challenge with a dose of 2 Gy. At 24 h post-2 Gy IR, cells were harvested, stained with Annexin V/PI and analyzed using a flow cytometer. Apoptosis is shown as values expressed as fold change relative to control (Ctrl). (d) Bar graph depicting the percentage of membrane-intact and membrane-damaged cells following 2 Gy exposure, with and without a 0.5 Gy priming dose. Data are means ± SEM (n = 2); *P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t-test.
