Fig. 5
In A549, cell death induced after a large 2 Gy challenge dose could be partly reversed by pre-treatment of cells with a dose of 0.5 Gy. (a) Representative flow cytometry dot plots of A549 cells after challenge with a dose of 2 Gy. At 24 h post-2 Gy IR, cells were harvested, stained with Annexin V/PI and analyzed using a flow cytometer. Apoptosis is shown as values expressed as fold change relative to control (Ctrl). (b) Bar graph depicting the percentage of membrane-intact and membrane-damaged cells following 2 Gy exposure, with and without a 0.5 Gy priming dose. (c) Reverse transcription-quantitative PCR results for the detection of HMOX1, SOD, Bcl2, IL-1β and TNF-α mRNA expression. Twenty-four hours after exposure to 0.5 Gy, A549 cells were harvested, and target genes expression relative to β-actin were determined by real-time PCR. Data are means ± SEM (n = 2); *P < 0.05, ***P < 0.001, ****P < 0.0001 by Student’s t test.
