Fig. 3

Activity of isoxazole 3 at 10 µM against 20 human and 3 viral cysteine proteases. The following were used as controls: E-64 (50 nM) Cathepsin B, K, L, S, and V; Cystatin C (300 nM) for Cathepsin F; Z-VRPR-FMK (10 nM) for MALT1; Ubiquitin-Aldehyde (0.1 nM) for UCHL3, (1 nM) for A20, UCHL1, USP1, USP14, (10 nM) for OTUD6B, USP2, USP5, USP7, USP8, USP10, USP20, and (1 µM) for Ataxin-3; GC376 (1 µM) for SARS2 Mpro; Protease Cocktail (10 µM) for MERS Mpro; and GRL0617 (10 µM) for SARS2 Plpro. Values are the average of duplicate determinations.