Fig. 5
Streptavidin pull-down in HEK293 lysates by VS and biotin-azide click reaction. (a) Silver stain of SDS-PAGE gel loaded with HEK293 lysate (lane L) or proteins from streptavidin pull-down of cell lysate alone (lane 1). The VS pull-down was performed 3 times from the lysate and loaded onto the gel: VS alone (lane 2), VS alone from lysate spiked with nsP2 protein (lane 3), VS from lysate pre-blocked with 3 (lane 4). An uncropped copy of the gel with membrane edges visible is shown in Supplementary Figure S22. (b) Analysis of proteins identified from lanes 2–4 by LC–MS/MS. All identified proteins (▲); VS alone (◼); VS after pre-block with 3 (◌); VS alone from lysate spiked with nsP2 protein (⬤). Proteins with log2 fold < 5 enrichment are shown in grey. Proteins with log2 fold > 5 enrichment are shown in black, with nsP2 in red.
