Fig. 8

Synthesis of clickable vinyl sulfone (VS) and pre-clicked vinyl sulfone control (TVS). Reagents and conditions: (i) diethyl oxalate, NaH; (ii) NH₂OH.HCl; (iii) 3-bromoprop-1-yne, K₂CO₃; (iv) NaOH; (v) (E)−3-(methylsulfonyl)prop-2-en-1-amine, TBTU, pyridine; (vi) 6-TAMRA azide, CuSO₄, Na ascorbate.